Erythropietic colonies in cultures of human marrow.
نویسندگان
چکیده
The cell culture method originally devised the total pool of human CFU-E in that they by Stephenson and Axelrad for erythrohave a slower mean sedimentation velocpoietin-dependent erythropoietic colony ity and a greater erythropoietin requireformation (CFU-E) by mouse cells has been ment. The assay is quantitative and was adapted to human marrow. Erythropoietic used to determine the radiation sensitivity colonies from human marrow contain beof human erythropoietic progenitors. The tween eight and 150 cells and reach maxiparameters of the survival curve are: =
منابع مشابه
BIO treatment enhances rat marrow-derived mesenchymal stem cell in vitro proliferation and viability
Introduction: Previous investigations have indicated that the presence of BIO (6-Bromoindirubin-3-Oxime) in medium of some cell culture enhances the cell proliferation and viability. The aim of the present study was to investigate the BIO effects on in vitro expansion of rat marrow-derived mesenchymal stem cells (MSCs) culture. Methods: In the present experimental study, bone marrow cells from ...
متن کاملEosinophil colony formation in semisolid cultures of human bone marrow cells.
A method for in situ staining and scoring of eosinophil colonies and clusters in the whole culture dish has been developed for agar cultures of human marrow cells. Cultures stimulated by human placental conditioned medium were found to develop the same proportion of eosinophil, neutrophil, and monocyte colonies as cultures stimulated by conventional peripheral white cell underlayers. Eosinophil...
متن کاملEffects of Lithium Chloride on In Vitro Proliferation Rate of Rat Marrow- Derived Mesenchymal Stem Cells
Purpose: To evaluate the effects of lithium chloride on MSCs in vitro expansion rate. Materials and Methods: In this experimental study, bone marrow from 8 rats was plated at 5×105- cells/cm2 in the presence of 1,2,5,7 and 10 mM Lithium chloride and expanded through 3 passages. Twelve days after initiatial culture, the cells of different groups were stained with crystal violet in order to compa...
متن کاملHuman marrow cells capable of erythropoietic differentiation in vitro: definition of three erythroid colony responses.
A systematic study has been undertaken to analyze the spectrum of erythropoietic colonies obtained in cultures of human marrow cells plated in methyl cellulose. Colonies were identified as erythropoietic on the basis of the appearance in them of hemoglobin-containing erythroblasts. As found previously in mouse marrow cultures, three sequentially appearing types of colonies which differed in the...
متن کاملStimulation of persisting colonies in agar cultures by sera from patients with CML and AML.
Cord plasma contains colony-stimulating activity (CSA) which stimulates the in vitro clonal growth of neutrophils, eosinophils, macrophages, erythrocytes, and persisting mast cells in semisolid cultures. Analysis of day 35 colonies in agar cultures was found to be a suitable means of demonstrating this activity and discriminating between it and granulocyte-macrophage colony-stimulating factor (...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Blood
دوره 44 5 شماره
صفحات -
تاریخ انتشار 1974